Hook up with hermaphrodite

come into association with their hermaphroditic counterparts they cease foraging behavior and begin to mate.

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If cues work only proximally, then movement by males outside regions conditioned with hermaphrodites should not be affected (Fig. In a modified version of the response assay, we observed the extent and direction of movement at various distances, using outwardly concentric areas from A–D, with respect to a conditioned point (Fig. To corroborate previous findings, we scored number of reversals and the time in region (now area A) in a manner similar to our response assay.More than 95% of males introduced to conditioned agar trials found their mates within 2 h; in contrast, only ≈77% of males introduced to unconditioned agar trials had found them at the same time point.Moreover, all of the males in conditioned agar trials found their mates, whereas ≈10% of males in unconditioned trials never found their mates within 5 h ( Organisms use a variety of mechanisms to orient and navigate toward chemical cues in their environment (33).Isolate trials were run in parallel alongside trials of the laboratory strain (Bristol N2).Unconditioned agar trials were meant to parallel conditioned agar trials as closely as possible: ( Design of response, holding, and attraction assays.